Retinal vascular permeability was assessed by measuring Evan s blue leakage into vitreous cavity. Finally, Epo was injected into the vitreous of murine retina, followed by intravenous injection of Evan s blue dye 6 hours later. Cell growth and migration was measured manually and using Cell Counting Kit 8 (Dojindo Molecular Technologies, Gaithersburg). Migration and proliferation assays were performed according to manufacture s instruction (Promocell, Germany Cambrex, Walkersville) on in vivo endothelial cells using increasing concentrations of Epo and VEGF. This procedure was repeated in control mice. Methods Used: After exposure to hyperoxic conditioning, murine vitreous samples were obtained at postnatal day 17, retina isolated and RNA extracted for Epo and VEGF mrna determination. We hypothesized that Epo and VEG-F mrna expression is up-regulated in oxygen induced retinopathy (OIR) and these proteins are involved in endothelial cell proliferation, migration and permeability. It is not known whether and how these growth factors are involved in ROP. Recent research in diabetic retinopathy, thought to have similar pathogenesis to ROP, has shown that erythropoietin (Epo) and vascular endothelial growth factor (VEGF) are involved in neovascular angiogenesis. Purpose of Study: Retinopathy of prematurity (ROP) is a prevalent morbidity among preterm infants and is characterized by retinal neovascularization. 1 University of Utah, Salt Lake City, UT 2 University of Utah, Salt Lake City, UT and 3 University of Utah, Salt Lake City, UT. 1 Journal of Investigative Medicine 56(1):, 2008 Ó 2008 by The American Federation for Medical Research W E S T E R N R E G I O N A L M E E T I N G A B S T R A C T S WAFMR, WSCI, WAP and WSPR Joint Plenary Session II 1:45 PM Thursday, January 31, EXPRESSION AND FUNCTION OF ERYTHROPOIETIN AND VASCULAR ENDOTHELIAL GROWTH FACTOR INCREASED IN OXYGEN INDUCED RETINOPATHY S.

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